The Spinning Disk Confocal Microscope enables high-speed and high resolution measurements as well as 3D imaging. The piezo sample holder allows X Y cross-sections with very narrow spacing in the Z plane. This way, 3D reconstructions of fluorescence distributions in living cells are possible for each time point. Visualization of dynamic processes within and of living cells is possible when investigating intracelluar transport or analyzing cell distributions in 3D scaffolds.